3 Credits 15 theoretical hours and 30 practical hours
OBJECTIVES:To provide the student, the vast majority of whom have a background in chemistry, with basic knowledge that is essential in evaluating the possible biological effects of new molecules with therapeutic potential. Presenting a practical approach to studying bioactivity in experimental models.
SUMMARY: Present the biosafety measures that involve experimentation with biological material. Show strategies for cell cultivation. Discuss the fundamentals of the techniques used in the analysis of biological activities of compounds with chemotherapeutic potential. Analyze the main techniques used to evaluate cytotoxicity, anti-inflammatory, anti-tumor and anti-parasitic action. Offer the opportunity to carry out a pilot test of the anti-parasitic activity of a selected compound.
PROGRAM CONTENT:
1- Biosafety1.1- Types of risks
1.2- Methods for controlling risk agents
1.3- Security booths
1.4- Procedures for disposing of waste generated in laboratories
1.5- Sterilization standards
2- Cell cultivation
2.1- Culture media
2.2- Primary cells, cell lines and transformed cells
2.3- Adherent and non-adherent cells
2.4- Cultivation of parasites
3- In vitro biological activity assays
3.1- Cytotoxicity
3.2- Anti-tumor activity
3.3- Anti-inflammatory activity
3.4- Anti-parasitic activity
BIBLIOGRAPHY:
01. Biosafety in Biomedical Laboratories and Microbiology portal.lanvisa.gov.br/wps/wcm/connect/802ba4804798d25d9f4ebf1leefca640/biosseguranca_em_laboratorios_biomedicos_e_de_microbiologia.pdf?MOD=AJPERES.
02. Molecular Biology of the Cell, 4th edition Bruce Alberts, Alexander Johnson, Juliean Lewis, Martin Raff, Keith Roberts, and Peter Walter, New York: Garland Science; 2002.
03. Cell Culture Basics – www.vanderbit,edu/viibre/cellculturebasicsEu.pdf
04. Clinical Parasitology: a clinical-laboratory approach. Elizabeth Zeibig. Elsevier.
