2 credits 30 theoretical hours
OBJECTIVE OF THE DISCIPLINE: To enable a broad scientific view of the most varied
Biochemistry topics from the researchers’ point of view and not from the topics studied.
SUMMARY: Bibliographic review of the main discoveries in the area of Biochemistry and Biology
Molecular
ANALYTICAL PROGRAM
1. Nucleic acids and genetic information
1.1. Structure of DNA – Rosalind Franklin
1.2. The discovery of introns – Richard J. Roberts and Philip A. Sharp
1.3. Fragments of Okasaki – Reiji Okasaki
2.Amino acids
2.1. Discovery of the 20th amino acid (threonine) – Willian C. Rose
2.2. Discovery of the 21st amino acid (selenocysteine) – A. Bock
2.3. Discovery of the 22nd amino acid (pyrrolysine) – Joseph Krzycki, John Atkins, Ray Gesteland
(2002)
3. Peptides and Proteins
3.1. Discovery of insulin – Frederick Banting and Charles Best
3.2. Protein sequencing – Frederick Sanger
3.3. Structure and Function of Hemoglobin – Max Perutz
4. Enzymes
4.1. Enzymatic action – J. B. S. Haldane
4.2. DNA polymerase – Arthur Kornberg
4.3. ATP synthase – John E. Walker
4.4. Polysaccharide (glycogen) phosphorylase – Carl and Gerty Cori
5. Metabolism
5.1. Study of metabolism – Otto Warburg
5.2. The Cori cycle – Carl and Gerty Cori
5.3. The tricarboxylic acid cycle – Hans Krebs
5.4. Chemiosmotic Theory – Peter Mitchell
5.5. Cellular fermentation – Eduard Buchner
5.6. The Calvin cycle – Melvin Calvin
5.7. Cholesterol biosynthesis – Konrad Bloch
5.8. The ornithine cycle – Hans Krebs
5.9. Biosynthesis of saccharides – Luis Leloir
Bibliography
McCoy, R.H.; Meyer, C.E.; Rose, W. C. (1935). J. Biol. Chem. 112, 283-302.
Perutz, M.F.; Rossman, M. G., Cullis, A. F.; Muirhead, H.; Will, G.; North, A.C.T. (1960). Nature, 185, 416-422.
Briggs, G. E.; Haldane, J.B.S. (1925) Biochem. J. 19, 339.
Warburg, O. (1956). Science, 123, 309-314.
Cori, G. T., Colowick, S. P., Cori, C. F. (1939). J. Biol Chem., 127, 771-782.
Krebs, H. A., Johnson, W. A. (1937). Enzymologia, 4, 148-156.
Mitchell, P. (1961). Nature, 191, 144-148.
Banting, F. G., Best, C. H. (1922) J. Lab. Clin. Med. 7, 251-266.
Franklin, R.E., Gosling, R.G. (1953). Nature, 171, 740-741.
Kornberg, A. (1969). Science, 163, 1410-1418.
Chow, L. T., Gelinas, R. E., Roberts, R. J. (1977). Cell, 12, 1-8.
Berget, D. M., Moore, C., Sharp, P. A. (1977). Proc. Natl. Academic. Sci., 74, 3171–3175.
Okazak,i R.; Okazaki, T.; Sakabe, K.; Sugimoto, K. (1967). Jpn J Med Sci Biol., 20(3), 255-260.
Atkins, J. F.; Gesteland, R. (2002). Science, 296 (5572), 1409–1410.
Krzycki, J. (2005). Curr. Opinion. Microbiol., 8(6), 706–712.
Zinoni, F.; Birkmann, A.; Stadtman, T. C; Böck, A. (1986). PNAS, 83, 4650-4654.
Calvin, M. (1956). Bull. Soc. Chim. Biol. 38 (11),. 1233–1244.
Bloch, K., Rittenberg, D. (1942) J. Biol. Chem. 145, 625-636
