3 Credits 45 Practical hours
OBJECTIVES: Allow the student to know and apply analytical techniques for the
isolation and purification of natural and synthetic substances.
SUMMARY:
1. Chromatographic Methods (14 hours).
2. Extraction, Separation and Purification Methods (16 hours)
3. Isolation and Purification of Organic Substances.
4. Reactions to characterize functional groups (16 hours)
PROGRAM CONTENT:
01. Chromatographic Methods
Chromatography (introduction): Mechanisms of adsorption, partition, ion exchange and exclusion.
Open column chromatography, analytical and preparative thin layer, paper. Types and Recovery
of adsorbents.
02. Introduction to gas chromatography, high performance liquid chromatography and introduction to
counter current chromatography.
03. Extraction, Separation and Purification methods applied to organic substances.
Liquid-liquid extraction, Soxhlet extraction, acid-base extraction. Crystallization. Sublimation.
04. Chemical Characterization: Functional Groups and Classes of Natural Products
05. Structural modifications to organic substances.
Reactions: Oxidation, reduction, methylation, demethylation, acetylation, hydrolysis, ketal formation, esterification
BIBLIOGRAPHY:
1. “Introduction to Experimental Phytochemistry” – F.J. Abreu Matos, UFCE, Fortaleza, CE, 1980.
“Principles and Practice of Modern Chromatographic Methods” – K. Robards, P.R. Haddad, P.E.
Jackson, Academic Press, Harcourt Brace & Company, Publishers, London, 1997.
2. “Practice of Thin Layer Chromatography” – J.C. Touchstone and M.F. Dobbins, John Wiley &
Sons, New York, USA, 1983.
3. “Introduction to Chromatographic Methods” – C.H. Collins and G.L. Braga, Ed. from UNICAMP,
Campinas, SP, 1988.
4. “Reactions and Synthesis in the Organic Chemistry Laboratory” – L.F. Tietze and T. Eicher,
Springer Verlag, Germany, 1988.
5. Textbook of Practical Organic Chemistry” – A. I. Vogel – B. S. Furniss; A. J. Hannaford; P. W.
G. Smith and A. R. Tatchell. Longman Scientific & Technical. 5th Ed.1991.
6. “Métodos de Investigación Fitoquímica”, Jorge A. Dominguez, Limusa, México, 1973.
7. Phytochemical Methods. A guide to Modern Techniques of Plant Analysis. J.B. Harborne,
Chapman and Hall, London. 1973.
